PICSI is advanced technique than basic ICSI. In ICSI the selection of sperms is dependent on embryologist criteria of selection. Here entire natural selection is over looked. PICSI, is a method where mature sperms with specific receptor binds on to the hyaluronic acid (HA). Immature sperms lack these receptors and are screened out with PICSI. The bound sperms indicate that they have completed the spermatogenesis cycle implying nuclear maturity and adopts method in natural process. With the aid of PICSI dish, partial selection of sperms are done and makes it easier for IVF specialist chose best sperms out of all the attached sperms. 


Here we have IVF treatment with ICSI and PICSI, which are assisted artificial method in IVF that introduces spermatozoon into the cytoplasm of oocyte. Intra cytoplasmic sperm injection (ICSI) commonly used technique for selection of best spermatozoa that helps in improving fertilization success rate. In this the selection of sperm is done by immobilizing them in PVP. Then manually selected spermatozoa is inserted in the cytoplasm of ovum   by kinking tail region. But Physiological ICSI (PICSI) uses hyaluronan selection of sperm which has reduced the liability in total pregnancy failure. It’s because selected sperm have low DNA damages or any chromosomal disorder in conception outcome. We at our IVF centre have specialists who provide a better prognosis for the male factor infertility by opting PICSI among the couples who had a failure in conception, poor grade embryo formation, miscarriages and recurrence of poor/no fertilizationalready undergone ICSI treatment.

Why PICSI is different?

 ICSI is the most preferred technique for in-vitro fertilization which helps in sorting viable and morphologically best sperm in the semen sample. However PICSI is an innovative method for selecting mature sperms by the use of hyaluronan which minimize the risk of pregnancy failure like miscarriage, stillbirth and congenital anomalies. Moreover, there is a routine semen sample and its functional tests done in andrology laboratory. We as an organization are connected to fulfill and satisfy all of our patient’s requirements with an enhanced set-up and a group of individuals and experts ensuring the best and accurate result. This not only changes the outcome of fertilization rate, but also helps in attaining treatment among offspring desiring couple by transferring good grade embryos that are fertilized in the embryology laboratory by an embryologist.

The process of fertilization initiates when male spermatozoon and female oocyte fuse with each other, resulting in zygote formation. Similar event occur in the IVF laboratory. There are diverse techniques developed in assisted conception fertilization aided by procedures such as IVF, ICSI and PICSI that is available in the best IVF center among which we stand to provide by ensuring good service with full concern and care to patient pre and post procedural. The advancement in PICSI process reduces the risk of pregnancy failure and good embryo formation by assessment of spermatozoon being an essential part before performing PICSI providing solution for problem in ART.

How PICSI is done in our embryology laboratory? PICSI procedure is similar to ICSI. PICSI is based on the use of Hyaluronic Acid (HA) which is a glycosaminoglycan which is biologically active molecule with high molecular weight. This is a major component found in the extracellular matrix found in OCC, forming a barrier against functionally deficient spermatozoa in the human body. Similarly, in-vitro fertilization were matured and fully developed spermatozoa binds to HA that is synonymous to binding with zona pellucida of oocyte in the fallopian tube (in vivo). An immature spermatozoa lack the receptor and showing an inability to bind with HA. The viable sperm has HA binding ability having intact and capacitated acrosome showing less chances of degraded DNA component. Our IVF specialist in Delhi use these selected sperms by PICSI dish to fertilize mature oocytes. PICSI fertilized embryos lead to good pregnancy outcomes here at our IVF Centre Delhi.

Fig: Oocyte showing Oolemma Layer
Fig: Oocyte showing Oolemma Layer