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What is PRP?
Platelets play a vital role in repairing local tissues and endometrium receptivity due to the presence of growth factors and various cytokines. In this procedure, patient’s own platelet enriched plasma is extracted in our IVF Centre by removing RBC’s from their own blood. Hence, it is considered as safe and natural treatment. The autologous concentration of plasma is essential for proliferation and differentiation of mesenchymal cells that help in the rebuilding of local tissues.
How it works?
The presence of growth factors and various cytokines helps to enhance autocrine and paracrine influence, which helps in local tissue remodeling and increases the endometrial receptivity for implantation.
The growth factors and cytokines present in the PRP:
Transforming growth factor-beta
Epidermal growth factor (EGF)
Interleukin 8 (IL-8)
Platelet-derived growth factor (PDGF)
Insulin like growth factor I and II
Vascular endothelial growth factor
Fibroblast growth factor
Connective tissue growth factor
Keratinocyte growth factor
How PRP sample is prepared?
- In our IVF laboratory, we collect fresh peripheral blood around 12ml with the help of a syringe. The blood is mixed with an anti-coagulant.
- Then the sample is processed by using the double centrifugation method and separates components of blood, i.e. RBCs, WBC’s and plasma from the whole blood.
- After first centrifugation, top layer platelet-rich plasma with growth factor and WBC’s are transferred to a fresh conical tube along with Buffy coat layer.
- After the second centrifugation, the platelet-rich plasma forms a pellet at the bottom of the tube. It is carefully separated from poor plasma, which is the upper layer.
- This process will increase the concentration of platelets to near about four times to the normal and the prepared platelet-rich plasma is injected inside the mother via different routes, depending upon the treatment required.
How PRP is performed?
PRP is performed by two techniques in our IVF Centre; one is Endometrial PRP and the second is Ovarian PRP1. Endometrial PRP: For a successful pregnancy, a significant relationship between mother and fetus is required in Assisted Reproductive Treatment. Despite of transferring a good quality embryo, it failed to implant due to low endometrial
receptivity or thin endometrial lining and that leads to recurrent miscarriage. The endometrial and embryonic development require receptors of growth factors, cytokines and various adhesion molecules for healthy endometrium. The IVF doctor in our center performs PRP therapy in the females diagnosed with thin endometrial lining and poor responders. Healthy endometrium consists of various growth factors and cytokines like epidermal growth factor, insulin like growth factor, transforming growth factor beta, growth hormone and platelet-derived growth factor. Therefore, the intervention with PRP infusion becomes an effective mediation to obtain optimal endometrial lining as these cytokines and growth factors will help in decidual vascularization and proliferation of the endometrium. This when accompanied with good quality embryos, it results in successful conception in the previous failed IVF cases.
2. Ovarian PRP: It is a laparoscopic-assisted approach and a promising treatment that helps to rejuvenate and regenerate the ovaries due to the presence of the growth factors and various cytokines to enhance the egg quality and production. It is performed in the cases where the females wanted to conceive with their own eggs but have failed IVF cycles due to low AMH, advanced maternal age, or if they are diagnosed with ovarian insufficiency. In such cases, PRP injections are given which is an ultrasound-guided procedure into the ovaries by our IVF specialist and thus, it helps in inducing change within a few months in the level of AMH hormone.